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Image Search Results
Journal: Scientific Reports
Article Title: Investigating the antifibrotic effect of the antiparasitic drug Praziquantel in in vitro and in vivo preclinical models
doi: 10.1038/s41598-020-67514-4
Figure Lengend Snippet: Praziquantel effects in the BioMAP diversity panel. Profile of PZQ effects on the Diversity PLUS Panel. Human primary cells in the systems are used at early passage (4 or earlier) to minimize adaptation to cell culture conditions and preserve physiological signalling responses. The x-axis lists the quantitative protein-based biomarker readouts measured in each system. The Y-axis represents a log-transformed ratio of the biomarker readouts for the PZQ-treated sample over vehicle controls. The grey region around the Y-axis represents the 95% significance envelope generated from historical vehicle controls. Biomarker activities are annotated when 2 or more consecutive concentrations change in the same direction relative to vehicle controls, are outside of the significance envelope, and have at least one concentration with an effect size > 20% (|log1- ratio| > 0.1). Biomarker key activities are described as modulated if these activities increase in some systems, but decrease in others. Cytotoxicitiy is indicated on the profile plot by a thin black arrow above the X-axis, and antiproliferative effects are indicated by a thick grey arrow. All cells are from a pool of multiple donors (n = 2–6), commercially purchased and handled according to the recommendations of the manufacturers. X-axis from left to right: CCL2/MCP−1; CD106/VCAM−1; CD141/Thrombomodulin; CD142/Tissue Factor; CD54/ICAM−1; CD62E/E−Selectin;CD87/uPAR; CXCL8/IL−8; CXCL9/MIG; HLA−DR; Proliferation; SRB; CCL2/MCP−1; CCL26/Eotaxin−3; CD106/VCAM−1; CD62P/P−selectin; CD87/uPAR; SRB; VEGFR2; CCL2/MCP−1; CD106/VCAM−1; CD141/Thrombomodulin; CD142/Tissue Factor; CD40; CD62E/E−Selectin; CD69; CXCL8/IL−8; IL−1alpha; M−CSF; sPGE2; SRB; sTNF−alpha; CCL2/MCP−1; CD38; CD40; CD62E/E−Selectin; CD69; CXCL8/IL−8; CXCL9/MIG; PBMC Cytotoxicity; Proliferation; SRB; B cell Proliferation; PBMC Cytotoxicity; Secreted IgG; sIL−17A; sIL−17F; sIL−2; sIL−6; sTNF−alpha; CCL2/MCP−1; CCL26/Eotaxin−3; CD106/VCAM−1; CD54/ICAM−1; CD90; CXCL8/IL−8; IL−1alpha; Keratin 8/18; MMP−1; MMP−3; MMP−9; PAI−I; SRB; tPA; uPA; CD54/ICAM−1; CD87/uPAR; CXCL10/IP−10; CXCL11/I−TAC; CXCL8/IL−8; CXCL9/MIG; EGFR; HLA−DR; IL−1alpha; Keratin 8/18; MMP−1; MMP−9; PAI−I; SRB; tPA; uPA; CCL2/MCP−1; CD106/VCAM−1; CD141/Thrombomodulin; CD142/Tissue Factor; CD87/uPAR; CXCL8/IL−8; CXCL9/MIG; HLA−DR; IL−6; LDLR; M−CSF; PAI−I; Proliferation; Serum Amyloid A; SRB; CCL2/MCP−1; CD106/VCAM−1; CD54/ICAM−1; Collagen I; Collagen III; CXCL10/IP−10; CXCL11/I−TAC; CXCL8/IL−8; CXCL9/MIG; EGFR; M−CSF; MMP−1; PAI−I; Proliferation_72hr; SRB; TIMP−1; TIMP−2; CCL2/MCP−1; CD54/ICAM−1; CXCL10/IP−10; CXCL8/IL−8; CXCL9/MIG; IL−1alpha; MMP−9; PAI−I; SRB; TIMP−2; uPA; alpha−SM ; Actin; bFGF; CD106/VCAM−1; Collagen I; Collagen III; Collagen IV; CXCL8/IL−8; Decorin; MMP−1; PAI−I; SRB; TIMP−1; CCL2/MCP−1; CCL3/MIP−1alpha; CD106/VCAM−1; CD40; CD62E/E−Selectin; CD69; CXCL8/IL−8; IL−1alpha; M−CSF; sIL−10; SRB; SRB−Mphg.
Article Snippet: Using another model of established fibrosis, PZQ, tested in the
Techniques: Cell Culture, Biomarker Assay, Transformation Assay, Generated, Concentration Assay